Myh6 cre mouse

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August undefined, 2024

WebTg (Myh6-icre/ERT2)#Wet. Mutation details : The mouse promoter drives cardiac-specific expression of a tamoxifen-inducible humanized cre (icre) fused to an estrogen receptor … Web24 mrt. 2024 · Toll-like receptor 4 (TLR4) is an integral factor in the initiation of the innate immune response and plays an important role in cardiovascular diseases such as hypertension and m

Abstract 13138: Dissection of Cardiomyocyte versus Fibroblast …

Web23 jul. 2015 · Abstract. A mouse model that mediates temporal, specific, and efficient myocardial deletion with Cre-LoxP technology will be a valuable tool to determine the … Web21 mei 2024 · Adult cardiomyocytes from the ventricles of Myh6-Cre GSDMD +/+ mice (controls) or GSDMD-CKO (cardiomyocyte-specific GSDMD-deficient) male mice were isolated, and ( A) H/R for 0.5/24 h induced the pyroptotic morphological manifestations of prominent bubble-like formations, as well as propidium iodide (PI)-positive staining of … tracy andrade

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Web16 aug. 2024 · The paper for the first time shows that MKK6 reduces life span in mice, and that young mice with MKK deficiency display cardiac hypertrophy and that it progresses to cardiac dilatation and fibrosis as they age. Web1 feb. 2024 · Myh6-Cre transgenic mouse line was known to express Cre recombinase only in the heart. Nevertheless, during breeding Myh6-Cre to Rosa26 fstdTom reporter … Web27 feb. 2024 · Cardiomyocyte-specific deletion of Tead1 ( Tead1 -cKO) was achieved by crossing Tead1F/F mice with Myh6- Cre mice (011038, The Jackson Laboratory). Mice were maintained under standard 12-h light-dark cycles with ad lib access to food and water, unless specified otherwise. the rox roxbury wisconsin

011038 - αMyHC-Cre Strain Details - The Jackson …

A1cf Transgenic Allele Detail MGI …

WebTo examine the role of aggregates in cardiomyopathy, mice with a perinatal onset cardiac selective (Myh6-Cre driven) deletion of an adaptor protein, p62, (p62cKO) were generated. WebThe WT, Myh6-Cre, and Mb21d1 −/− mice had normal survival, but, as reported previously, the Myh6-Cre:Lmna F/F mice exhibit total mortality within the first 4 weeks of life with maximum and median survival rates of 25 and 20 days, respectively . 17 Deletion of the Mb21d1 gene prolonged the maximum and median survival rates of the Myh6-Cre:Lmna … theroxtonprogress.comWeb7 jul. 2024 · Adult cardiomyocytes from the ventricles of Myh6-Cre GSDMD +/+ mice (controls) or GSDMD-CKO (cardiomyocyte-specific GSDMD-deficient) male mice were … the roxton apartments denton

"WebMyh6 Kcnj8 Tg(Myh6-Kcnj8)634Kcmm GH: Transgene: Diabetes and Obesity Research Cardiovascular Research ... Cre/loxP system: RBRC10016. B6.129S2-Rhoj/76Maim: Rhoj neo: Targeted Mutation: RBRC10015. B6.Cg-Bcl6 loxP Bcl6 EYFP: ... Mouse Models for Human Disease " - Myh6 cre mouse

Myh6 cre mouse

Generation and characterization of a Myh6-driven Cre …

WebGenotyping primers for these two new alleles are listed in table S5. Generation and genotyping of the Nkx2.5 cre and Myh6 mercremer lines has been described previously (9, 35, 36). Mice were kept on a mixed C57BL/6:129SVJ background. The experimental MI procedure is described in Supplementary Materials and Methods. WebOnline Methods Animal work Mesp1-Cre1, Tbx1-Cre2, Islet1-Cre3, Hcn4-GFP4,5, Wntless loxp (stock no. 012888, Jackson Laboratory6), and Ai9 (stock no. 007909, Jackson Laboratory7) mouse strains were utilized in in vivo experiments. Embryos were harvested from embryonic day 8.0-9.5 for further analysis. For evaluating the phenotype of each …

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Web5 mrt. 2024 · Myh6-MerCreMer:LARP7 f/f mice were injected with 25 mg/kg/d tamoxifen (iCKO) for continuous 5 days at 2 months of age to deplete LARP7 from cardiomyocytes. Two-month-old MerCreMer mice with the same tamoxifen injection plan were used as controls (n=6 to 7; 2-tailed Student t test; ** P <0.01). Web30 jun. 2024 · Mice expressing constitutively active Cre recombinase driven by the Myh6 promoter [B6.FVB-Tg (Myh6-cre)2182Mds/J] were obtained from The Jackson Laboratory. Mice containing a floxed (flanking lox P) Gdf11 allele with lox P sites flanking exons 2 and 3 of Gdf11 were generously provided by Dr. Se-Jin Lee (Johns Hopkins University) ( 13 ).

Web南模生物可提供超过200种自主产权Cre工具鼠和近500种自主产权成品Flox小鼠，有效缩短您的实验周期。南模生物部分Cre品系验证数据 Myh6-Cre. Myh6特异性靶向小鼠心肌细胞，验证数据证明Myh6-Cre鼠是可以用于心脏特异性基因编辑的工具鼠。图13. Web条件性敲除小鼠定义：条件性基因敲除小鼠（也叫Flox小鼠）是指在目的基因中含有成对的loxp位点的小鼠，与Cre工具小鼠交配后可在特定的组织或细胞中敲除目的基因。lCKO如何实现？重组酶系统（如：Cre-loxP）介导的位点特异性重组技术。Cre是重组酶（38kDa），可识别34bp长的DNA序列loxP。

Web靶基因信息：Cre recombinase，Cre (Cyclization Recombination Enzyme,即环化重组酶）是来源于噬菌体P1的一种酶蛋白，分子量约38kDa。. 它可以识别催化两个LoxP位点之间发生同源重组，从而造成DNA的缺失、易位等现象。. 构建信息：该转基因在小鼠心脏特异性α-肌球 … Web15 sep. 2024 · Inducible Cre recombinase using Myh6 promoter has also been established [17, 18]. However, adverse effect of tamoxifen treatment on normal heart function in …

Web18 aug. 2024 · And it was reported that the Myh6-Cre mice with similar but mixed genetic background displayed no cardiotoxicity (26, 27). In present study, in order to avoid the potential cardiotoxicity from Myh6-Cre, the mating strategy to generate Myh6-Cre-BDNF flox mice with mixed background was adopted as shown in methodology.

Web009074 STOCK Tg(Myh6-cre)1Jmk/J These transgenic mice express nuclear-localizing Cre recombinase under the control of the mouse Myh6 (myosin, heavy polypeptide 6, … the rox tampaWeb18 okt. 2024 · 而Cre-loxP位点特异性重组系统（简称Cre-loxP重组系统）的建立，为完善基因编辑小鼠模型的构建，进一步开展条件性靶基因功能的研究，提供了有效工具。. 回顾Cre-loxP重组系统在小鼠研究中的实际应用，研究者们认识到，对该技术应用中存在的某些风险与 … tracy andre hardaway seattle waWebMice were received standard chow and water, and maintained on an alternating 12-h light-dark cycle. Stim1 flox/flox (Stim1 f/f; B6 (Cg)-Stim1 tm1Rao /J; stock no. 023350) mice and MHC-cre mice (B6.FVB-Tg(Myh6-cre) 2182Mds … tracy andreen hallmarkWeb7 mrt. 2024 · We next characterized the hearts of adult Myh6-Cre;Thra DN /+ mutant mice and their regenerative capacity . Adult thyroid hormone mutant hearts were 37% larger than controls ( Fig. 4B and fig. S16) and contained an increased number (approximately twofold) of cardiomyocytes as shown with both dissociation ( Fig. 4C ) and stereological methods … the rox tasmaniaWebAbstract. Discovering determinants of cardiomyocyte maturity and the maintenance of differentiated states is critical to both understanding development and potentially reawakening endogenous regenerative programs in adult mammalian hearts as a therapeutic strategy. the roxton aptsWeb14 mei 2024 · Here, we develop Myh6-Cre double KO (DKO) ( Mbnl1−/−; Mbnl2cond/cond; Myh6-Cre+/−) mice to eliminate Mbnl1/2 in cardiomyocytes and observe spontaneous lethal cardiac events under no anesthesia. RNA sequencing recapitulates DM heart spliceopathy and shows gene expression changes that were previously undescribed in DM heart studies. the roxton dentonWeb24 jan. 2024 · Title: Myh6-driven Cre recombinase activates the DNA damage response and the cell cycle in the myocardium in the absence of loxP sites. A New Mouse Model of … tracyandrusfoundation.com