Jc-1 red/green ratio
WebSep 1, 2011 · Due to perfect alignment of the two images, we established an online ratiometric analysis of JC-1 fluorescence (ratio = JC-1 red *5/JC-1 green; the factor “5” transformed the ratiometric results into an 8-bit scale of 256 levels). WebJC‐1 does not form aggregates in mitochondria’s with low ∆ψ M and remain in monomeric form and exhibit green fluorescence. Thus higher the ratio of red to green fluorescence, …
Jc-1 red/green ratio
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WebJC-10 is developed to be a superior alternative to JC-1 when high dye concentration is desired. Compared to JC-1, JC-10 has much better water solubility. JC-10 is capable of selectively entering mitochondria, and reversibly changes its color from green to orange as membrane potentials increase. WebCompared to shNT cells, shUSF3 cells showed significantly higher JC-1 red-green ratio indicating mitochondrial hyperpolarization (Fig. ... View in full-text Context 4
WebJC-1 and JC-9 are cationic dyes (Figure 1) that exhibit potential-dependent accumulation in mitochondria, indicated by a fluorescence emission shift from green (~525 nm) to red (~590 nm). Consequently, mitochondrial depolarization is indicated by a decrease in the red/ green fluorescence intensity ratio. WebMay 10, 2012 · Flying ability (D and E), ATP levels (F), Ψ m at neuromuscular boutons determined by using JC-1 red/green ratio (G), and mitochondrial morphology in third instar larval muscles (H to J) in control pink1 RV and in pink1 B9 on MK-4 containing medium (gray) or control medium (black) [(D) to (I)] or upon overexpression of Heix (da>heix) (J). …
WebMar 30, 2016 · Cells were harvested and stained with MitoTracker Deep Red (MTDR) (A), JC-1 (B), or MitoTracker Green (MTG) (C), followed by flow cytometric analysis. The ratio of the red to green fluorescence intensity in JC-1 stained cells was used to represent mitochondrial membrane potential. WebOct 23, 2013 · B, FCCP reduced the MMP in vivo as indicated with the JC-1 ratio (red/green) in Wt mouse cortex. FCCP (10 μ m) was applied topically to the cortex for 15 min. The ratio of J-aggregate (red) to J-monomer (green) changed in …
WebMay 22, 2006 · The aggregated form of JC-1 molecules (red fluorescence) accumulated in functional mitochondria. On the other hand, the green fluorescence signal from JC-1 monomers is evenly distributed in the cytoplasm (Fig. 1 A and B).In the control groups, the red/green ratio was 18.2 ± 9.3, while in the hypoxia/re-oxygenization group this ratio was …
WebJC-1 mitochondrial potential measurement was analyzed on a LSRII flow cytometer where a 488 nm excitation laser was used in combination with FITC and PE emission channels to measure green and red fluorescence of the JC-1 stain, respectively. The measured results were analyzed using the FlowJo software and compared with that of Cellometer imaging login my tetraWebJan 26, 2004 · The fluorescent signal intensity at 590 nm (red) was divided by the intensity at 535 nm (green), and this red/green ratio was used to estimate the mitochondrial membrane potential (ΔΨ m ). Larger JC-1 red/green … login my tesco accountWebMitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates. Ideal for detection of collapse of ΔΨ m induced by uncouplers of mitochondrial respiration. JC-1 has also been used in a ... log in my texas benefitsWebmtSOX Deep Red is a novel mitochondrial superoxide probe, designed for multiple staining with high selectivity towards superoxide [New] Lysosomal Acidic pH Detection Kit This kit includes lysosome staining dyes, pHLys Red (pH dependent), and LysoPrime Green (pH-independent). Lysosomal pH and lysosomal mass can be measured by combining these … login mythriventWebWhen using JC-1 dye for mitochondrial membrane potential we get a decrease in the green fluorescence at the same time as a reduction in the red. The reduced red is easily … login my texas benefitslog in my texas medicaidWebJC-1 and JC-9 are cationic dyes (Figure 1) that exhibit potential-dependent accumulation in mitochondria, indicated by a fluorescence emission shift from green (~525 nm) to red … login my three