Cytochalasin b glucose uptake
WebApr 14, 2016 · Glucose uptake for GlcP Se was measured one minute after initiation of transport with 30 μM C 14 -glucose in the presence of 2 mM … WebCytochalasin B has been shown to potently inhibit the transport of glucose, deoxyglucose, and glucosamine by Novikoff hepatoma cells in suspension culture without affecting their …
Cytochalasin b glucose uptake
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WebNov 10, 2014 · To further confirm our findings in primary brown adipocyte cultures, glucose uptake in BAT was measured in vivo in β 1 /β 2 knockout (to exclude any effects on β 1 - or β 2-adrenoceptors) and wild-type FVB mice that express all three β-adrenoceptor subtypes (Fig. 4, A and B). Isoproterenol increased glucose uptake in BAT in vivo in FVB and ... WebEnter the email address you signed up with and we'll email you a reset link.
WebIn Fig. 4 (right side), we summarize the re- sults of 4 -6 independent experiments, comparing the insulin-dependent net change in cytochalasin B binding sites with the net change in GLUT4 glucose ... WebFeb 27, 2024 · The present study was conducted to explore the mechanism of nano-Zn absorption and its influence on lipid metabolism in the intestine of yellow catfish Pelteobagrus fulvidraco. Compared to ZnSO4, dietary nano-Zn addition increased the triglyceride (TG) content, enzymatic activities of malic enzyme (ME) and fatty acid …
WebThis dependence results in up-regulation of glucose transporter expression, especially hGLUT1. The bound inhibitors include the natural compound cytochalasin B and two of a series of previously undescribed organic compounds that bind in the submicromolar range. WebMar 1, 1990 · Glucose uptake in muscle occurs by a system of facilitated diffusion involving at least two distinct glucose transporters, GLUT-1 and GLUT-4. Both bind the fungal …
Web2.2 Assaying Insulin-Stimulated Glucose Uptake. 1. Serum-free media: DMEM (with 4.5 g/L glucose, l-glutamine, and sodium pyruvate) containing 0.5 % bovine serum albumin …
WebCytochalasin B (17·3 μ M) virtually abolished 3- O -methyl- D - [U- 14 C]glucose uptake and D - [5- 3 H]glucose utilization in tumoral insulin-producing cells of the RINm5F line. This coincided with a marked decrease in D - [U- 14 C] glucose oxidation and suppression of the stimulant action of D -glucose upon insulin release. dam definition geographyWebThe widely expressed mammalian glucose transporter, GLUT1, can be acutely activated in L929 fibroblast cells by a variety of conditions, including glucose deprivation, or treatment with various respiration inhibitors.Known thiol reactive compounds including phenylarsine oxide and nitroxyl are the fastest acting stimulators of glucose uptake, implicating … mario 64 falling screamWebRCME cells exhibited stereospecific hexose uptake inhibited by cytochalasin B. Pretreatment of the RCME cells with potassium cyanide or 2,4-dinitrophenol inhibited 2-deoxyglucose uptake but not 3-O-methylglucose transport. A major proportion (30–60%) of the 2-deoxyglucose present in the RCME cells was not phosphorylated. mario 64 ds versionWebMay 13, 2024 · Insulin stimulates glucose uptake by acutely upregulating GLUT4 surface levels mainly through increased exocytosis of GSVs and decreased endocytosis 33, 34. A single GLUT4 molecule during its... dam cuoi viet hanWebMay 10, 1972 · A mold metabolite, cytochalasin B, inhibits uptake of 2-deoxy-d-glucose in chick fibroblasts and cultured liver cells. This inhibition is rapid, reversible, and is … dam cuoi o viet namWebJun 18, 2024 · The glucose transporter GLUT1, a plasma membrane protein that mediates glucose homeostasis in mammalian cells, is responsible for constitutive uptake of glucose into many tissues and organs. Many studies have focused on its vital physiological functions and close relationship with diseases. mario 64 ea versionWebNov 11, 2008 · Cytochalasin B (10 μmol/L) and phloretin (20 μmol/L) inhibited glucose uptake 85 ± 1% and 84 ± 18%, respectively. In contrast, the selective GLUT4 inhibitor … dam di boccanera