http://wolfson.huji.ac.il/purification/PDF/detergents/SIGMA_DetergentsBiofiles.pdf Webthe Web site. Use the CelLytic M reagent component of this kit as a lysis buffer. Cell Lysis Notes for cell lysis • The volume of CelLytic™ M reagent to add to the cells varies with the cell size and protein concentration required. In general, 125 µL of CelLytic™ M are recommended for 106-107 cells.
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WebCelLytic™ M is compatible with Coomassie®Blue and silver staining of gels. Protein lysates can also be used for DNA-protein interaction assays (gel-shift assays). CelLytic™ M … WebUse of CelLytic Y reagent involves a short procedure (20 − 30 minutes) performed at room temperature using standard lysis conditions (i.e. mild detergent and pH 8) without the need for extreme conditions or glass beads required by other methods. CelLytic Y can be used for extraction of proteins from fresh or frozen yeast cells. The cell ... in a multitude of counselors there is wisdom
Product Information CelLytic™ M - Sigma-Aldrich
WebZTJT $ CelLytic M, Cell Lysis Reagent, suitable for Mammalian Cell Lysis and Protein Solubilization 10 mL, 40 mL,50 mL, 60 mL,250 mL, 1L $&CelLytic Mem Protein Extraction Kit, for membrane proteins 1Kit $ CelLytic Mt Cell Lysis Reagent, for Mammalian Tissues 50 mL, 500 mL /953"$5CelLytic NuCLEAR Extraction Kit, for Mammalian Tissue Or Cultured … Webactivity), and 150 mM NaCl. CelLytic MT can also be used for the efficient extraction of cell-line proteins that require salt in the lysis buffer. For cells not requiring salt in the lysis … WebCelLytic™, Sigma’s suite of reagents used for bacterial cell lysis and extraction of solubilized recombinant proteins, as well as mammalian and plant cell lysis. An extensive table of the physical properties and recommended biological applications for key detergents. BioUltra detergents for demanding applications, where in a multitude of words